Structural Determinants of Salmon Calcitonin Bioactivity
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منابع مشابه
Structural Determinants of Salmon Calcitonin Bioactivity: the Role of the Leu-based Amphipathic Α-helix*
Giuseppina Andreotti, Blanca López Méndez Pietro Amodeo, Maria A. Castiglione Morelli, Hiromichi Nakamuta and Andrea Motta From the Istituto di Chimica Biomolecolare del CNR, Comprensorio Olivetti, Edificio A, 80078 Pozzuoli (Napoli), Italy; the Dipartimento di Chimica, Università della Basilicata, 85100 Potenza, Italy; and the Laboratory of Pharmacology, Department of Pharmaceutical Sciences, ...
متن کاملStructural determinants of salmon calcitonin bioactivity: the role of the Leu-based amphipathic alpha-helix.
Salmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central...
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When freshwater eels were given either 5 or 15 i.u. calcitonin kg" body weight, there was an increase in dorsal aortic blood pressure as well as a rise in urine volume, glomerular filtration rate and electrolyte excretion rate, 1 h after injection. None of these changes was shown by seawater-adapted eels. When freshwater-adapted eels received 15 i.u. calcitonin kg" body weight there were signif...
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A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After affinity purification, the antibodies were used both as immobilized capture an...
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The therapeutic activity of peptides or protein drugs is highly dependent on their conformational structure. The protein structure is flexible and responds to external conditions, which may compromise the protein’s native conformation and influence its physical and chemical stability. The physical and chemical stability of peptides or protein drugs are important characteristics of biopharmaceut...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m603528200